IMPROVEMENT OF REPRODUCTIVE EFFICIENCY BY ADENOSINE INJECTION IN MALE RABBITS

Document Type : Original Article

Authors

1 Department of Animal Biotechnology, Animal Production Research Institute, Dokki, Giza, Egypt.

2 Department of Zoology, Faculty of Science, Tanta University, Egypt .

3 Department of Zoology, Faculty of Science, Kafr El-shiekh University, Egypt

Abstract

This study was designed to evaluate mature New-Zealand White (NZW)male rabbits physiological and reproductively as affected by different levels of adenosine as growth hormone on reproductive functions of male rabbits. Eighteen white New Zealand mature male rabbits aged 5-6 months and weighing (2.7-2.9 kg) were divided into three groups (6 rabbits each). First group of rabbits served as a control group, second and third rabbit groups were injected intravenously by (0.4 and 0.8 mg of adenosine/ animal/48hours) respectively, dissolved in 0.5 ml (0.9 NaCl) for 60 days. Blood samples were taken once to evaluate blood components and semen was collected twice to evaluate physical semen characteristics from each animal every 10 days.
Best results were recorded in the second group of animals (injected with 0.4 mg adenosine/animal/48 hours), where the mean was (7.8gm/dL) for total serum protein, (14.5gm/dL) for hemoglobin, (9.8gm/dL)for calcium concentration and (7.05nmol/L) for testosterone hormone level that increased significantly (P<0.05) compared to other groups at the end of the experiment. Also, the results the second group animals presented (0.97 ml ) for semen ejaculating volume, (85%) for sperm motility, (91.8%) for sperm live that significantly increased at (P<0.05) compared to other groups and showed a significant reduction of (6%) for sperm abnormalities than those found in other groups of animals at 60 days of experimental time.
Conclusively, this study shows that the injection by 0.4 mg adenosine/animal every 48 hours for 60 days continuously improved physical semen characteristics, blood components and can safely be used on rabbit males.
 

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